Hypothesis: Ingestion of two grams of Rejuvamin combined with water by athletic males and females of ages 26 to 38 years, four times a day for six weeks on an empty stomach, will significantly increase pituitary growth hormone production as reflected by an increased plasma level of insulin-like growth factor-one (IGF- 1), without raising liver enzyme SGOT or SPOT in either sex, or PSA in males.

Testing Methods: One hundred and sixty-eight two-gram packages of Rejuvamin were bundled with a patient diary and distributed to a population of exceptionally fit male and fernale athletes** A baseline series of blood chemistries was performed. The subjects were instructed to self-administer the Rejuvamin preparation on an empty stomach four times a day for six weeks. The suggested regimen was:

• first packet immediately upon waking from sleep in the morning
• second two hours after breakfast and at least one hour before ingesting anything else
• third packet two hours after lunch mid at least one hour before ingesting anything else
• fourth packet at least two hours after dinner and preferably immediately before going to bed

We instructed the subjects to make a notation in the diary each time they ingested the preparation. The subjects were to perform their exercise routines as usual

The Results:

.	IGF-1 START	IGF-1 FINISH	% CHANGE	CHANGE IN LIVER FUNCTION	CHANGE IN PSA
MEN 1 2 3 4 5 6 WOMEN 1 2* 3 4 5 6	106 120 146 124 162 108   182 350 125 90 124 116	241 200 276 165 225 196   241 283 275 120 208 128	+127.4 + 66.7 +89.0 +33.1 +38.9 +81.5   +32.4 -19.1 +120.0 +33.3 +67.7 +10.3	NONE NONE NONE NONE NONE NONE   NONE NONE NONE NONE NONE NONE	NONE NONE NONE NONE NONE NONE

Conclusion: The ingestion of two grams of Rejuvamin combined with water by athletic males and females of ages 26 to 38 years, four times a day for six weeks on an empty stomach, significantly increased pituitary growth hormone production as reflected by an increased plasma level of insulin-like growth factor one (IGF-1). The average change in males was +72.8 percent and the average change in females was +40.9 percent, in no single case was there a rise in liver enzyme SGOT or SPOT in either sex, or PSA in males.

**Defined as burning 1500 calories per week in exercise and with Less than 10% body fat.

SEX	AGE	START	2 MONTHS	4 MONTHS	6 MONTHS
F	57	101	140	143	177
F	53	112	143	158	308
M	74	131	146	187	212
M	49	142	161	179	263
M	63	122	146	172	246
M	82	144	162	213	262
M	45	138	186	194	281
F	40	141	192	186	267
F	65	106	148	173	242
M	49	134	172	211	245
F	58	129	153	166	*
M	60	133	156	188	307
F	49	118	134	171	*
M	48	124	139	162	170
AVERAGE	56.6	126.8	155.6	178.8	248.3
% CHANGE FROM BASELINE	.	.	+22.7%	+41.0%	+95.0%
* not yet reported

Clinical Study carried out by:
Dr. Walter B. Essman
927 Park Avenue, New York, NY 10028
phone 212-452-2255
fax 212-452-4522

Several orally administered amino acids, either individually or in combination, have been shown to elevate human growth hormone (HGH) levels as a consequence of the acute stimulation of HGH release from the anterior pituitary gland. A mixture containing the amino acids L-glutamine, L-lysine HC1, and L-arginine, as well as sugars, citric acid and natural flavoring, has been evaluated for HGH secretagogue potential.

Thirteen patients (six females and seven males) showed pre-treatment baseline IGF-1 levels, a plasma marker for HGH secretion, of 117.83 ng/ml (+/- 6.66) females (median age=55) and 134.57 ng/ml (+/- 2.00) males (median age=49). After 60 days of treatment with the proprietary preparation, three doses per day on an empty stomach at least 45 minutes before mealtimes, IGF-1 levels were elevated by 25% to 156.85 ng/ml (+/-4.40). After four months, IGF-1 levels had increased by 46% over baseline to 181.25 ng/ml (+/- 8.75), and by six months, IGF-1 levels had risen by 100.1% above pre-treatment levels to 255.4 ng/ml (+/-31.00).

These data have consistently suggested that the amino acid formulation used (Rejuvamax® from Biocentrics, Brentwood, TN 37024-1063) is an effective HGH secretagogue. No adverse side effects were noted and patient acceptance was excellent.

Hypothesis: Ingestion of five grams of Rejuvamin Plus combined with water by athletic males of ages 26 to 38 years, four times a day for three weeks on an empty stomach, will significantly increase pituitary growth hormone production as reflected by an increased plasma level of insulin-like growth factor-one (IGF-1), without raising liver enzyme SGOT or SPOT, or PSA.

Testing Methods: Eighty-four five-gram packages of Rejuvamin Phis were bundled with a patient diary and distributed to a population of exceptionally fit male athletes* A baseline series of blood chemistries was performed. The subjects were instructed to self-administer the Rejuvamin Plus preparation on an empty stomach four times a day for three weeks. The suggested regimen was:

• first packet immediately upon waking from sleep in the morning
• second two hours after breakfast and at least one hour before ingesting anything else
• third packet two hours after lunch and at least one hour before ingesting anything else
• fourth packet at least two hours after dinner and preferably immediately before going to bed

We instructed the subjects to make a notation in the diary each time they ingested the preparation. The subjects were to perform their exercise routines as usual.

The Results:

	IGF-1 START	IGF-1 FINISH	% CHANGE	CHANGE IN LIVER FUNCTION	CHANGE IN PSA
MEN 1 2 3 4 5 6	241 204 169 125 156 146	356 259 225 207 254 197	+ 47.7 + 27.0 + 33.1 + 65.6 + 62.8 +34.9	None None None None None None	None None None None None None